Tc13tul antigen from Trypanosoma cruzi induces B-cell proliferation in splenocytes from naive BALB/c mice

TASSO, LM; BRUBALLA, AC; GARAVAGLIA, PA; ESTEVA, MI; ALBAREDA, MC; GARCIA, GA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias de la Republica Argentina

Several soluble factors of Trypanosoma cruzi, the ethiological agent of Chagas´ disease, modulate host immune responses, among them the enzyme trans-sialidase (TcTS), a member of group I of the trans-sialidase superfamily (TS). Here, we evaluated the effect of Tc13Tul antigen on splenocytes from naive BALB/c mice. Tc13Tul belongs to group IV of TSs, characterized by C-terminal EPKSA repeats.Splenocyte proliferation were assessed by CFSE staining after 72 h of incubation with recombinant Tc13Tul, EPKSA repeats or the carrier protein MBP as a control. Flow cytometry analysis showed an increase in the % of divided cells when incubated with Tc13Tul (11.65 ± 0.07 % vs. 0.93 ± 0.12 % for EPKSA or 1.29 ± 0.05 % for MBP; p