Galectin-1 Prevents Infection and Damage Induced by Trypanosoma cruzi on Cardiac Cells

BENATAR, A; GARCIA, GA; BUA, J; JUAN CERLIANI; POSTAN, M; TASSO, LM; SCAGLIONE, J; STUPIRSKI, JC; TOSCANO, MA; RABINOVICH, G; GOMEZ, KA

PLOS NEGLECTED TROPICAL DISEASES

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2015 vol. 9 p. 1 - 23

1935-2735

Background: Chronic Chagas cardiomyopathy caused by Trypanosoma cruzi is the result of a pathologicprocess starting during the acute phase of parasite infection. Among different factors, thespecific recognition of glycan structures by glycan-binding proteins from the parasite or fromthe mammalian host cells may play a critical role in the evolution of the infection. Methodology and Principal Findings: Here we investigated the contribution of galectin-1 (Gal-1), an endogenous glycan-bindingprotein abundantly expressed in human and mouse heart, to the pathophysiology of T. cruzi infection, particularly in the context of cardiac pathology. We found that exposure of HL-1cardiac cells to Gal-1 reduced the percentage of infection by two different T. cruzi strains,Tulahuén (TcVI) and Brazil (TcI). In addition, Gal-1 prevented exposure of phosphatidylserine and early events in the apoptotic program by parasite infection on HL-1 cells. These effects were not mediated by direct interaction with the parasite surface, suggesting that Gal-1 may act through binding to host cells. Moreover, we also observed that T. cruzi infection altered the glycophenotype of cardiac cells, reducing binding of exogenous Gal-1 to the cell surface. Consistent with these data, Gal-1 deficient (Lgals1-/-) mice showed increased parasitemia, reduced signs of inflammation in heart and skeletal muscle tissues,and lower survival rates as compared to wild-type (WT) mice in response to intraperitoneal infection with T. cruzi Tulahuén strain. Conclusion/Significance: Our results indica te th at Gal-1 modulates T. cruzi infection of cardiac cells, highlighting therelevance of galectins and their ligands as regulators of host-parasite interactions