INVESTIGADORES
VIZOSO PINTO Maria Guadalupe
artículos
Título:
Nasal priming with immunobiotic Lactobacillus rhamnosus modulates inflammation-coagulation interactions and reduces influenza virus-associated pulmonary damage
Autor/es:
ZELAYA, H.; TADA, A.; VIZOSO PINTO, M. G.; SALVA, S.; KANMANI, P.; AGÜERO, G.; ALVAREZ, S.; HARUKI, K.; VILLENA, J.
Revista:
INFLAMMATION RESEARCH
Editorial:
BIRKHAUSER VERLAG AG
Referencias:
Lugar: BASEL; Año: 2015
ISSN:
1023-3830
Resumen:
Objective To evaluate the effect of the nasal administration
of live and heat-killed Lactobacillus rhamnosus
CRL1505 (Lr1505) on immune-coagulative response during
influenza virus (IFV) infection to improve survival and
reduce lung injury.
Methods Six-week-old BALB/c mice were treated with
live or heat-killed Lr1505 by the nasal route during two
consecutive days. Treated and untreated control mice were
then nasally challenged with IFV.
Results Both viable and non-viable Lr1505 protected
infected mice by reducing pulmonary injury and lung
viral loads trough several mechanisms: (a) Inflammatory
cytokines were efficiently regulated allowing higher
clearance of virus and reduction of inflammatory lung
tissue damage, associated to higher levels of the regulatory
cytokine IL-10. (b) The antiviral immune response
was enhanced with improved levels of type I interferons,
CD4+IFN-gamma+ lymphocytes, and lung CD11c+CD11blowCD103+
and CD11c+CD11bhighCD103- dendritic cells.
(c) The procoagulant state was reversed mainly by downregulating
tissue factor expression and restoring thrombomodulin
levels in lung. The capacity of Lr1505 to
improve the outcome of IFV infection would be related to
its ability to beneficially modulate lung TLR3-triggered
immune response.
Conclusions Our work is the first to demonstrate the
ability of an immunobiotic strain to beneficially modulate
inflammation?coagulation interactions during IFV infection.
Interestingly, non-viable L. rhamnosus CRL1505 was
as effective as the viable strain to beneficially modulate
respiratory antiviral immune response.