INVESTIGADORES
VIZOSO PINTO Maria Guadalupe
artículos
Título:
Varicella Zoster Virus Infection of Malignant Glioma Cell Cultures: A New Candidate for Oncolytic Virotherapy?
Autor/es:
LESKE, HENNING; HAASE, R.; RESTLE, F.; SCHICHOR, C.; ALBRECHT, V.; VIZOSO PINTO, M. G.; TONN, J.C.; BAIKER, A.; THON, N.
Revista:
ANTICANCER RESEARCH
Editorial:
INT INST ANTICANCER RESEARCH
Referencias:
Año: 2012 vol. 32 p. 1137 - 1144
ISSN:
0250-7005
Resumen:
Background: Glioblastoma multiforme is a highly aggressive tumor with a
median survival of 14 months despite all standard
therapies. Focusing on alternative treatment
strategies, we evaluated the oncolytic potential of varicella zoster
virus (VZV)
in malignant glioma cell cultures. Materials and
Methods: Replication of wildtype and mutant VZV was comparatively
analyzed
in glioma cell lines (U87, U251 and U373) and in
primary malignant glioma cells (n=10) in vitro by infectious foci assay,
immunofluorescence microscopy and western blot
analysis. Additionally, the tumor-targeting potential of VZV-infected
human
mesenchymal stem cells was evaluated. Results: VZV
replicated efficiently in all the glioma cells studied here followed by
rapid oncolysis in vitro. The attenuated vaccine
VZV mutant rOKA/ORF63rev[T171] exhibited most efficient replication.
Human
mesenchymal stem cells were found suitable for
targeting VZV to sites of tumor growth. Conclusion: VZV exhibits an
intrinsic
oncolytic potential in malignant glioma cell
cultures and might be a novel candidate for virotherapy in glioblastoma
multiforme.