LuMPIS – A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein–protein interactions encoded by GC-low ORFs

VIZOSO PINTO, M. G.; VILLEGAS, J. M.; PETER, J.; HAASE, R.; HAAS, J.; LOTZ, A. S.; MUNTAU, A. C.; BAIKER, A.

PROTEOMICS (WEINHEIM. PRINT)

WILEY-V C H VERLAG GMBH

Año: 2009 vol. 9 p. 1 - 6

1615-9853

The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein–protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems.