NOTCH PATHWAY ROLE IN ANGIOGENESIS REGULATION IN BOVINE MAMMARY GLAND.

BONADEO, NADIA; PERRI, ADRIÁN; MEJÍA, MIGUEL; LICOFF N; BECU VILLALOBOS D; CAROLINA CRISTINA; ISABEL MARÍA LACAU

Buenos Aires

Jornada; Jornada. XVIII Jornadas Anuales Multidisciplinarias.; 2016

ROLE OF NOTCH PATHWAY IN ANGIOGENESIS REGULATION IN BOVINE MAMMARY GLANDBonadeo N1, Perri A2, Mejía M2, Licoff N2, Becú D2, Cristina C1, Lacau I M2 1-CIBA-CITNOBA, Newbery 261, Junín, province of Bs. As.; 2- IBYME, Obligado 2490, CABA. E-mail: nadia_bonadeo@hotmail.comNotch pathway is associated with stem cells maintenance, proliferation, angiogenesis and cell differentiation. These processes occur in bovine mammary gland development. Gastrointestinal parasites affect Holstein heifer development. Hereby we studied Notch receptor expression (Notch 1-4) and its role in angiogenesis along development in control (C) and anthelminthic drug treated (T) heifer mammary glands. We determined Notch 1-4 and the endothelial cells marker CD34 expression by immunohistochemically studies. We observed that these proteins are expressed in glandular parenchyma and in stromal cells at different stages of heifer development. We studied Notch receptor expression levels in its active (80 KDa) and membrane (110 KDa) forms by Western blot and we found increased the Notch 3 active domain and a reduction of Notch 2 active and membrane domains at 40 weeks of age. Notch 4 receptor expression decreased in heifers at 30 and 40 weeks of age. Moreover we determined Hes-1 mRNA expression, a target gen of the Notch pathway, and we found an increment of this gen expression at 40 weeks of age. Real Time studies showed that the expression of the endothelial cell markers VEGF and Endocan increased at 40 weeks of age. The expression of Notch 1-4 receptors and Hes-1 target gen suggest an activated Notch pathway in bovine mammary gland. The increase in VEGF (p= 0,014) and Endocan mRNA levels, high Notch 3 expression (p= 7,86E-07) and low Notch 2 (p=3,23E-05) and 4 (p=0,032) expression at 40 weeks of age suggests that Notch 2, 3 and 4 might be involved in angiogenesis regulation at that time of development.