INVESTIGADORES
DRAGHI Walter Omar
congresos y reuniones científicas
Título:
OCCURRENCE AND BIODIVERSITY OF BURKHOLDERIA SPP. IN NATURAL ENVIRONMENTS OR NON-TILLAGE SOILS OF ARGENTINA
Autor/es:
DRAGHI, W.O.; DEGROSSI, J.; ABDIAN, P.L.; SOLER BISTUÉ, A.; WALL, L.G.; ZORREGUIETA, A.
Lugar:
Prague
Reunión:
Congreso; International Burkholderia cepacia Working Group Meeting; 2011
Institución organizadora:
International Burkholderia cepacia Working Group
Resumen:
The Burkholderia
genus is a ubiquitous bacterial group occupying diverse niches in the
environment, such as soils, plants or water sources, and displaying a wide
range of biological features. Some of these activities include
phytopathogenicity, plant growth promoting, endophytic or symbiotic nitrogen
fixation, biological control of fungi and siderophore production (1). Although the
clinical relevance of some strains is associated to the Burkholderia cepacia complex (Bcc),
species comprise in this group showed relevant biological properties for plant
health and growth. In the context of the BIOSPAS global project (2), a survey
to recognize different Burkholderia
strains in soils with different agricultural management within the main agricultural
production area of Argentina was performed. Cultivable Burkholderia spp. on PCAT medium were identified by both Random
Fragment Length Polymorphism (RFLP) with HaeIII
digestion of an 869 bp recA amplicon
and sequencing of a 384 bp recA
fragment, according to Payne et al
(3). Most of the isolates belonged to species from the Bcc, mainly B. ambifaria (55%) and B. cepacia (14%) strains. RFLP dendograms
showed different patterns for Burkholderia
isolates that belong to the same species, suggesting gene polymorphism or,
alternatively the presence of new species that require a more detailed
characterization. Although the quantification did not show any statistical difference
among sites or treatments (reaching ca. 106 per gram of soil
irrespective of soils types or agricultural managements) we observed
differences in biodiversity indexes (Rarefaction, Simpson evenness Index), especially
when comparing non-cultivated vs. cultivated soils. Further studies about the Burkholderia evolution in these soils will
provide insight into the impact of agricultural management on the biodiversity
of this genus.