Antitumoral effect of IL-12 gene transfected via liposomes into

LUCÍA SPERONI; JULIETA GASPARRI; VICTORIA BUSTUOABAD; NADIA CHIARAMONI; A. SMAGUR; S. STANISLAW; MARÍA CRISTINA TAIRA; SILVIA DEL VALLE ALONSO

Acta Biochimica Polonica

Polish Biochemical Society- Polish Academy of Sciences- Committee of Biochemistry and Biophysics

Lugar: Polonia; Año: 2009 vol. 56 p. 1 - 5

Murine melanoma B16FO cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survivain vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host surviva