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Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several 18 rhizobia species express the enzyme ACC deaminase, which degrades the ethylene 19 precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of 20 ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this 21 gene is under the activity of the NifA-RpoN-dependent promoter thus, it is only 22 expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was 23 integrated into the M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 23 integrated into the M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 21 gene is under the activity of the NifA-RpoN-dependent promoter thus, it is only 22 expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was 23 integrated into the M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 23 integrated into the M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. M. loti has a gene encoding ACC deaminase, but this 21 gene is under the activity of the NifA-RpoN-dependent promoter thus, it is only 22 expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was 23 integrated into the M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 23 integrated into the M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. M. loti structural gene ACC deaminase (acdS) was 23 integrated into the M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. M. loti chromosome under a constitutive promoter activity. The 24 resulting strain induced the formation of a higher number of nodules and was more 25 competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. Lotus japonicus and L. tenuis. These results 2 26 suggest that the introduction of the ACC deaminase activity within M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry. M. loti in a 27 constitutive way could be a novel strategy to increase nodulation competitiveness of the 28 bacteria, which could be useful for the forage inoculants industry..

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