INVESTIGADORES
MASUH Hector Mario
congresos y reuniones científicas
Título:
Aedes aegypti larvae respond to Natural and Synthetic Odorants.
Autor/es:
PAULA V. GONZALEZ; PAOLA A. GONZÁLEZ AUDINO; HÉCTOR M. MASUH
Reunión:
Conferencia; International Chemical Ecology Conference (ICEC) 2013; 2013
Institución organizadora:
International Society of Chemical Ecology
Resumen:
The ability to detect a wide range of sensory cues is essential for the
survival and vectorial capacity of mosquitoes. This investigation focuses on
the analysis of olfactory-driven behavior in dengue vector mosquito Aedes aegypti (L.) larvae to respond to synthetic or natural odorant
stimuli.
We established a
sensitive olfaction-based behavior assay, following the technique previously established [1]. In this assay, the
distribution of 100 2nd or 3nd instar larvae of Ae.
aegypti was monitored to a range of odorants stimuli as well as appropriate
negative controls throughout a 60 minute time course. The number of larva in both
test and control zone was counted throughout all time-point and performance
index (PI) at 40min was
calculated to represent the
response characteristics of each odorant with +1 indicative of full attraction
while ?1 represents complete repulsion.
The
widely used insect repellent N, N-diethyl-m-toluamide (DEET), consistently
evoked highly significant negative PI as well as Indole and Acetophenone. When
using yeast extract, a know food source, the larvae responded with a
significantly positive PI as well as 2-Methylphenol, 1-Octen- 3-ol,
3-Meyhylphenol and fish food.
We also found that, consistent with the olfactory function, ablation of the larval antennae
eliminated these behavioral responses. It is important to study the behavior of
attractant and repellency, as it could contribute to the understanding of the mechanism of olfaction in Ae aegypti larvae.
[1] Yuanfeng Xia, et
al. (2008). PNAS, 105 (17): 6433- 6438.