Enantioselective response of olfactory receptors of the mosquito Aedes aegypti to major components of Eucalyptus grandis x Eucalyptus camaldulensis essential oils.

PAULA V. GONZALEZ; PAOLA A. GONZÁLEZ AUDINO; HÉCTOR M. MASUH

Congreso; I Congreso latinoamericano de Ecología Química; 2010

Dengue is an acute viral infection transmitted by mosquitoes Aedes aegypti (L.) and Aedes albopictus (Skuse). Mosquito control by ethnobotanical products is an important activity in studies of dengue. Crude botanical extracts, essential oils and others plants components are studied and used in different areas of insect pests control including the application as insecticides and mosquito repellents. Previous studies of our laboratory [1] established the composition of the interspecific hybrid Eucalyptus grandis x Eucalyptus camaldulensis essential oil by gas chromatography-mass spectrometry, determining the presence of 1,8-cineol at a rate of 49.65%, α - pinene in 30.65%, α-terpineol in 4.05%, p-cymene in 2.77%, trans- pinocarveol in 2.75%, linalool acetate in 1.43%, borneol in 1.42% and β - pinene at 1% as main components. Chromatographic conditions with chiral column were optimized for the separation of optical isomers of major components of E. grandis x E. camaldulensis essential oil. Enantiomeric composition was determined for components with optical activity: α – pinene, β – pinene, trans-pinocarveol and α-terpineol. Gas chromatography coupled to electroantennographic detection (GC-EAD) was used in Ae. aegypti to determine if any of the major components of essential oil under study elicit a signal in mosquito antennae. The results showed electrophysiological activity for 1,8-cineol and the (-) - α – pinene isomer. Electroantennography (EAG) technique was performed to confirm electrophysiological activity of the major components of the essential oil under study. Studies of electrophysiological activity of individual components of the oil are presented here.