Influence of SINAL7 in vegetative parameters in Arabidopsis

PERALTA, DA; DIEGO FABIAN GOMEZ CASATI; BUSI, M. V.

Córdoba

Congreso; . LII Reunión de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2016

SAIB

Seven in absentia like 7 gene (At5g37890, SINAL7)from Arabidopsis thaliana encodes a RING finger protein belonging to theSINA superfamily with E3 ubiquitin ligase activity. In previous work wedescribed the biochemistry characteristics of SINAL7 protein and itsself-ubiquitination capability in vitro.Moreover, previous evidence led us to propose the participation of SINAL7 in ahypothetical signalling pathway together with cytosolicglyceraldehyde-3-phosphate dehydrogenase in Arabidopsis.In this work we face the study of SINAL7 invivo in Arabidopsis knock-out and overexpressing SINAL7plants to find out its influence on plant physiology by the phenotypic analysisof the plants. Results showed that SINAL7 may be participating in theregulation of several vegetative parameters including biomass content,senescence and drought tolerance. In summary, we found that different levels ofSINAL7 in Arabidopsis may alter plantbiomass. In addition, we describe the possible compensatory effect of SINAL7counterparts in knock-out mutant sinal7 plants. Finally, we show theinfluence of SINAL7 in the complex process of senescence and in droughttolerance.