INVESTIGADORES
GOMEZ CASATI Diego Fabian
congresos y reuniones científicas
Título:
Increased expression of genes involved in Fe-S cluster biosynthesis in AtFH deficient plants.
Autor/es:
LEADEN, L; TUROWSKI, V.; MALIANDI, M. V.; BUSI, M. V.; MURGIA, I; DIEGO FABIAN GOMEZ CASATI
Lugar:
Budapest, Hungria
Reunión:
Congreso; 15th International Symposium on Iron Nutrition and Interactions in Plants.; 2010
Resumen:
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Iron-Sulfur
cluster are cofactors of proteins involved in central biochemical pathways and
their assembly, and the maturation Fe-S proteins requires complex cellular
machineries. There are three different systems for the biogenesis of bacterial
Fe-S proteins: The NIF system for specific maturation of nitrogenase in
azototrophic bacteria; and ISC assembly and SUF system for the generation of
housekeeping FeS proteins under normal and oxidative-stress conditions,
respectively. In eukaryotes, mitochondria are the primary site of cluster
formation of most Fe-S proteins. Assembly of the Fe-S cluster is mediated by the
ISC machinery (Iron-Sulphate Cluster
assembly). In our laboratory we have caracterized different genes and proteins
that are postulated to be involved in the Fe-S cluster biosyntesis in Arabidopsis thaliana. AtNfs1 (Cysteine
desulphurase, sulphur donor), AtFH (frataxin, iron donor); Isu1 (Scaffold
protein); HscB (co-chaperone) and HscA (heat shock protein). In this work we
evaluate the effect of the deficiency of AtFH in the expression of the genes
mentioned above. We measured the transcript level of these genes by QPCR in
mutants plants of A. thaliana, knock down (atfh-1) and antisense (as-atfh). All analized transcripts were
increased (2 to 8-fold). However, some iron-sulfur proteins such as aconitase
or succinate deshidrogenase were decreased in its catalytic activity. Our
results are in agreement with a role of frataxin in the biogenesis of Fe-S
proteins in plants.