INVESTIGADORES
GOMEZ CASATI Diego Fabian
artículos
Título:
A simply method for the addition of rotenone in Arabidopsis thaliana leaves.
Autor/es:
MALIANDI, M. V.; RIUS, S.; BUSI, M. V.; DIEGO FABIAN GOMEZ CASATI
Revista:
Plant Signaling and Behavior
Editorial:
Taylor & Francis
Referencias:
Año: 2015 vol. 10 p. 10738711 - 10738713
ISSN:
1559-2316
Resumen:
A simple and reproducible method for the treatment of Arabidopsis thaliana leaves with rotenone is presented. Rosette leaves were incubated with rotenone and Triton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures after rotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented an inhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.
