INVESTIGADORES
ESPINOSA Maria Beatriz
congresos y reuniones científicas
Título:
A Mouse model to assess "in vivo" apoptotic induction during embryo development.
Autor/es:
WILLIS M A; ALBAMONTE MS.; JENSEN F.; WILENSKY L; VITULLO AD; ESPINOSA MB
Lugar:
Buenos Aires
Reunión:
Congreso; Advances in the Care and Use of Laboratory Animals, International/Regional Meeting; 2004
Institución organizadora:
ICLAS; FESSACAL; AACyTAL; SoMeVe; ACMAL
Resumen:
The study of the effect of apoptotic drugs on embryo development would greatly facilitate by establishing appropriate mouse models. We tested the feasibility of pregnant females to analyze apoptosis in the developing embryo. Apoptosis induction was experimentally approached by in utero administration of pro-apoptotic drugs in day 7 of pregnancy. Pro-apoptotic drugs assayed were: sodium nitroprusiate (NPS) as NO donor, colchicine, and NMDA. Control as performed by injecting PBS alone. Drug effect was monitored 24 h after injection by general morphological inspection of embryos. Abortions were registered. We determined optimal drug concentration in each case. Degree and distribution of apoptosis was assessed by TUNEL in histological embryo sections.
