Experimental approach for "in utero drug administration in mouse

WILLIS M A; ALBAMONTE MS.; JENSEN F; WILENSKY L; VITULLO AD; ESPINOSA MB

Buenos Aires

Congreso; Advances in the Care and Use of Laboratory Animals, International/Regional Meeting; 2004

ICLAS; FESSACAL; AACyTAL; SoMeVe; ACMAL

Mouse animal models will enable us to simulate clinical conditions in addressing questions about the effects of pro-apoptotic drugs activity on pregnant females and embryos. Balb-c females were mated with males and the vaginal plug (vp) was taken as day 1 of pregnancy. The reproductive performance in our laboratory  showed  43% successful mating and 51.5% of females with vp were pregnant. The litter size was 8.7 (range: 1-20) showing that the strain has been lost inbreeding. The surgery was performed on 23 pregnant females, 200 embryos were recovered. In  52 (26%) of them  necrosis or abortive processes  was observed as result of the surgery (same success rate described in the literature). After having the  surgery  proved, we tested “in vivo” pro-apoptotic drugs. Mouse animal models will enable us to simulate clinical conditions in addressing questions about the effects of pro-apoptotic drugs activity on pregnant females and embryos. Balb-c females were mated with males and the vaginal plug (vp) was taken as day 1 of pregnancy. The reproductive performance in our laboratory  showed  43% successful mating and 51.5% of females with vp were pregnant. The litter size was 8.7 (range: 1-20) showing that the strain has been lost inbreeding. The surgery was performed on 23 pregnant females, 200 embryos were recovered. In  52 (26%) of them  necrosis or abortive processes  was observed as result of the surgery (same success rate described in the literature). After having the  surgery  proved, we tested “in vivo” pro-apoptotic drugs.