Identificación de los componentes cultivables del consorcio bacteriano degradador de hidrocarburos “M10”.

MESTRE MC, VÁZQUEZ SC, RUBERTO L, MAC CORMACK, WP

Buenos Aires

Simposio; VI Simposio Argentino y III Latinoamericano sobre investigaciones Antárticas; 2007

Instituto Antártico Argentino

The geographic isolation of Antarctica determines that in-situ bioremediation processes were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gasoil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to Sphingobacteriaceae family were identified.