Identificación de los componentes cultivables del consorcio bacteriano degradador de hidrocarburos “M10”.

MESTRE MC; VÁZQUEZ SC; RUBERTO L; MAC CORMACK, WP

Actas de VI Jornadas Nacionales y III Latinoamericanas de Comunicaciones sobre Investigaciones Antárticas

Instituto Antartico Argentino

Lugar: Buenos Aires; Año: 2007 p. 1 - 5

1851-555X

The geographic isolation of Antarctica determines that in-situ bioremediation processes were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to in-situ bioremediation processes were the most adequate alternative to reduce hydrocarbon pollution. To do so, it is required to have microorganisms with high degradation capacity and capable to survive to the harsh Antarctic environmental conditions. In this work we attempted to identify the cultivable components of the psychrotolerant hydrocarbon-degrading consortium M10, which is used in the development of in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and to in-situ bioremediation processes in Antarctica. M10 was cultured in different C sources (gas-oil or phenanthrene) and its components were identified following 2 strategies: 1) morphological and metabolic characterization following identification using commercial systems; 2) identification by sequencing the 16S rRNA genes. Using these methodologies isolates belonging to genera Pseudomonas and Stenotrophomonas and toPseudomonas and Stenotrophomonas and to Sphingobacteriaceae family were identified.family were identified.