Candida albicans triggers the caspase-1 pathway during infection in central nervous system(CNS)

MAYOL, GONZALO FEDERICO; VIGEZZI, CECILIA; CEJAS, HUGO; ICELY, PAULA ALEJANDRA; SOTOMAYOR, CLAUDIA ELENA

Los Cocos. Cordoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunologia

Interleukin-1β is an important cytokine involved in the control of the fungus Candida albicans. This cytokine is predominantly produced by the innate immune cells through the recruitment and activation of intracellular proteins complex called inflammasome, which controls the activation of caspase-1 and the cleavage of proIL-1β, followed by release of the active mature IL-1β. The aim of this work is to study the IL-1β Caspase-1 pathway in the CNS during infection by C. albicans. C57BL/6 mice were infected intravenously with 2.5 x106 C. albicans (ATCC 36801), and at 12, 24, 48 and 72 hours were sacrificed and their brains were removed to assess: fungal burden (UFC), histological changes (PAS and HE stain), expression of Caspase-1 and IL-1β (IF) and in situ production of cytokine (ELISA). At 12h after inoculation, the fungus was recovered from the brain of infected animals (p