Assessment of epithelial cell´s immune response to Staphylococcus aureus exposed to chitosan polysaccharide

PORPORATTO C, FELIPE V, ICELY PA, MORGANTE C; CORREA SG

Lima

Congreso; X Congreso de la Asociación Latinoamericana de Inmunología ALAI 2012; 2012

Asociación Latinoamericana de Inmunologia

Assessment of epithelial cells´ immune response to Staphylococcus aureus exposed to chitosan polysaccharide. Porporatto, Carina; Felipe, Verónica, 1Icely, Paula; Morgante, Carolina; 1Correa, Silvia. Instituto de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Villa María, Argentina; 1CIBICI (Conicet), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina Bovine mammary epithelial cells (bMEC) contribute to the innate immune response to intramammary infections. An effective strategy to prevent S. aureus infection could be to block bacterial adhesion to bMEC and promote the innate immune response by the epithelium. Chitosan (Ch) is an polysaccharide with antibacterial effects and ability to modulate the immune response. Herein we studied the modulatory activity of the Ch in bMEC. We evaluated the ability to modify the adherence and internalization of V329 S. aureus strain in bMEC, using the MACT cell line. MACT cells were incubated in the presence of Ch (0.01 to 100 ug/ml) for 24 h, and the adherence of V329 to the cells decreased by 80% in presence of 10 ug/ml Ch (CFU count, p