Trajectory reconstruction of breast differentiation

DANIELA SENRA; NARA GUISONI; LUIS DIAMBRA

Conferencia; Human Cell ATLAS General Meeting; 2021

Human Cell ATLAS

There is evidence of a hierarchical organization in the adult mammary epithelium where stem cells give rise to luminal and basal myoepitelial cell types. scRNA-seq has vastly broadened the horizons for understanding cellular differentiation. Stem cells identification is typically done using known stemness markers, which in sc-RNAseq technology are not always reliable due to dropout. Here we propose a new methodology based on Protein-Protein Interaction networks to identify stem/progenitor cells in human breast cells. We inferred the differentiation process using these cells as the origin of the trajectory to compute the pseudotime with the diffusion maps technique.