INVESTIGADORES
BAROLI Irene Mabel
congresos y reuniones científicas
Título:
Spectral and thermodynamic characterization of cytochrome b in stroma vesicles from spinach chloroplasts
Autor/es:
BAROLI, IRENE; FERNÁNDEZ-VELASCO, JAVIER G.; CROFTS, ANTONY R
Lugar:
San Francisco
Reunión:
Congreso; Annual Meeting of the Biophysical Society of America; 1991
Institución organizadora:
Biophysical Society of America
Resumen:
PS I enriched vesicles prepared by digitonintreatment of spinach chloroplasts have beencritically studied in terms of their content ofcytochrome b. In contrast with previous results(Peters et nj.,BBA 722:460-470,1983), cytochromeb6 showed both a spectral and thermodynamicheterogeneity (b-564, E,7--170+10mV and b-563,7--50+10 mV) and on ahe other hand we foundtEAt the cytochrome described as b-559 (Em -32mV) has actually its alpha peak at 560 nm aftd anEm 7-50+10 mV. No considerable amounts of eitherios (E 7-120 mV) or high potential (E 7-350mV) cyNbchrome b-559 could be detecte%. Thestoichiometry b-564:b-563:b-560:b-559L:b-559HinPS I enriched vesicles was 1:1:1.4:
