INVESTIGADORES
GAMBERALE Romina
artículos
Título:
A critical role for Syk protein tyrosine kinase in Fc receptor-mediated antigen presentation and induction of dendritic cell maturation
Autor/es:
SEDLIK CRISTINE; ORBACH D; VERON P; SCHWEIGHOFFER E; COLUCCI F; GAMBERALE ROMINA; IOAN FACSINAY A; VERBEEK S; RICCIARDI-CASTAGNOLI P; BONNEROT C; TYBULEWICZ VL; DI SANTO J; AMIGORENA SEBASTIAN
Revista:
JOURNAL OF IMMUNOLOGY
Editorial:
AMER ASSOC IMMUNOLOGISTS
Referencias:
Año: 2003 vol. 170 p. 846 - 852
ISSN:
0022-1767
Resumen:
Dendritic cells (DCs) are the only APCs
capable of initiating adaptive immune responses. The initiation of
immune responses requires that DCs 1) internalize and present Ags; and
2) undergo a differentiation process, called "maturation", which
transforms DCs into efficient APCs. DC maturation may be initiated by
the engagement of different surface receptors, including certain
cytokine receptors (such as TNFR), Toll-like receptors, CD40, and FcRs.
The early activation events that link receptor engagement and DC
maturation are not well characterized. We found that FcR engagement by
immune complexes induced the phosphorylation of Syk, a protein tyrosine
kinase acting immediately downstream of FcRs. Syk was dispensable for DC
differentiation in vitro and in vivo, but was strictly required for
immune complexes internalization and subsequent Ag presentation to T
lymphocytes. Importantly, Syk was also required for the induction of DC
maturation and IL-12 production after FcR engagement, but not after
engagement of other surface receptors, such as TNFR or Toll-like
receptors. Therefore, protein tyrosine phosphorylation by Syk represents
a novel pathway for the induction of DC maturation.