INVESTIGADORES
GAMBERALE Romina
artículos
Título:
Acidosis improves uptake of antigens and MHC class I-restricted presentation by dendritic cells
Autor/es:
VERMEULEN MÓNICA; GIORDANO MIRTA; TREVANI ANALIA; SEDLIK CRISTINE; GAMBERALE ROMINA; PAULA FERNANDEZ CALOTTI; SALAMONE GABRIELA; RAIDEN SILVINA; JULIETA SANJURJO; GEFFNER JORGE
Revista:
JOURNAL OF IMMUNOLOGY
Editorial:
AMER ASSOC IMMUNOLOGISTS
Referencias:
Año: 2004 vol. 172 p. 3192 - 3204
ISSN:
0022-1767
Resumen:
It is widely appreciated that
inflammatory responses in peripheral tissues are usually associated to
the development of acidic microenvironments. Despite this, there are few
studies aimed to analyze the effect of extracellular pH on immune cell
functions. We analyzed the impact of acidosis on the behavior of
dendritic cells (DCs) derived from murine bone marrow. We found that
extracellular acidosis (pH 6.5) markedly stimulated the uptake of
FITC-OVA, FITC-dextran, and HRP by DCs. In fact, to reach similar levels
of endocytosis, DCs cultured at pH 7.3 required concentrations of Ag in
the extracellular medium almost 10-fold higher compared with DCs
cultured at pH 6.5. Not only the endocytic capacity of DCs was
up-regulated by extracellular acidosis, but also the expression of
CD11c, MHC class II, CD40, and CD86 as well as the acquisition of
extracellular Ags by DCs for MHC class I-restricted presentation.
Importantly, DCs pulsed with Ag under acidosis showed an improved
efficacy to induce both specific CD8(+) CTLs and specific Ab responses
in vivo. Our results suggest that extracellular acidosis improves the
Ag-presenting capacity of DCs.