INVESTIGADORES
GAMBERALE Romina
artículos
Título:
Medroxyprogesterone acetate enhances in vivo and in vitro antibody production
Autor/es:
VERMEULEN MÓNICA; PAZOS P; LANARI C; MOLINOLO A; GAMBERALE ROMINA; GEFFNER JORGE; GIORDANO MIRTA
Revista:
IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2001 vol. 104 p. 80 - 86
ISSN:
0019-2805
Resumen:
In the present study we examine the
effects of medroxyprogesterone acetate (MPA) on the specific antibody
secretion to T-dependent antigens. Our results show that the in vivo
administration of MPA to mice, 7 or 90 days before immunization with
sheep red blood cells (SRBC), significantly enhanced both, primary and
secondary antibody responses, without affecting delayed-type
hypersensitivity (DTH). These effects could be counteracted by the
anti-progestin onapristone or ZK 98299 (ZK) suggesting that MPA
interacted with progesterone (PRG) receptors to increase B-cell
response. To better understand the mechanisms involved in MPA activity
we carried out cultures of splenocytes, bone marrow cells or lymph node
cells from immunized mice in the presence of MPA, and evaluated the
amount of antibody release to supernatants. We found that low doses of
MPA (10(-9) M and 10(-10) M) significantly enhanced the in vitro
production of specific immunoglobulin G (IgG) antibodies, an effect that
appears to involve the interaction of the progestin with PRG receptors,
as judged by the inhibition of MPA effects with ZK (10(-8) M) or RU486
(10(-9) M). These receptors were detected by flow cytometry analysis in a
proportion of T lymphocytes. Because MPA did not increase the number of
immunoglobulin-secreting cells, our findings suggest that MPA enhanced
the capacity of individual cells to produce specific immunoglobulin.