PLASMA FINGERPRINTS ACQUIRED BY MALDI- TOF MS AS MARKERS OF PATHOPHYSIOLOGICAL EVOLUTION IN SEPSIS

LAUTARO MACEIRA; M. FLORENCIA TODERO; LEDESMA, MARTÍN; CARLOS VAY; BEATRIZ LÓPEZ; MONICA PRIETO; YOKOBORI, NOEMI; BÁRBARA REARTE

CABA

Congreso; Reunión Anual de Siociedades de Biociencias; 2020

SAIC SAI SAFIS

Sepsis constitutes one of the main causes of death in ICUs, presenting a bi-phasic nature with an inflammatory phase followed by an anti-inflammatory response. However, clinically, it?s difficult to define a therapeutic approach due to a lack of tools to determine the phase a patient is going through. Our aim was to investigate the usefulness of plasma fingerprints as instrument for discrimination of pro or anti-inflammatory states in sepsis using MALDI-TOF technology. For this, we used cecal ligation and puncture (CLP) murine models, which develop to an inflammatory phase during the 2nd day (D2) and an anti-inflammatory one on the 4th day (D4). The SHAM (SH) group was used as control. To characterize the different phases, hematologic, metabolic and immunologic parameters were determined in plasma. Also, plasma spectra were acquired by the MALDI-TOF technique. Un-supervised (Hierarchical K-means clustering) and supervised analyzes were performed to discriminate the different phases from differential signals (peaks). Peripheral blood leukocytes count was decreased in CLPD2 whereas in CLPD4 was increased associated to neutrophilia (Leukocytes (109/l): SHD2=7.7±0.4; CLPD2=3.5±0.6*. Neutrophils: SHD4=0.47±0.11; CLPD4=2.59±0.43&, &*p