ALTERED NEUTROPHIL ACTIVATION IN ABSENCE OF IL-10 IN A MURINE MODEL OF HEMOLYTIC UREMIC SYNDROME

GONZALO EZEQUIEL PINEDA; BÁRBARA REARTE; MARLINA CÓRDOBA MORENO; ANDREA BRUBALLA; ROMINA FERNANDEZ-BRANDO; ROBERTO MEISS; MARTIN ISTURIZ; CATALINA ALBA-SOTO; MARINA PALERMO; MARIA VICTORIA RAMOS

Mar del Plata

Congreso; Reunión Anual Conjunta SAIC-SAI-SAFE; 2016

Hemolytic Uremic Syndrome (HUS), is a systemic disease caused by circulating Shiga-toxin (Stx) which mainly damageskidney. Besides Stx2, inflammatory response mediated by neutrophils(PMN) is essential to HUS evolution. We have demostrated that mice lacking IL-10 (IL-10-/-) had a higher survival rate to Stx2 than controls.The aim of the work was to determine the role of PMN in HUS evolutionin absence of IL-10.Before and 24h, 48h and 72h after of 1LD100ofStx2 e.v., IL-10-/- and control mice were sacrificed. Total leukocytes were counted and PMN were identified as Ly6G+CD11b+ cellsin blood and bone marrow. PMNproduction of reactive oxygen species (ROS) was evaluated without stimulus (basal) or after PMA stimulation, with DHR by FACS.In basal condition both strains showed similar absolute number of PMN in blood, although the percentage of PMN in bone marrow was increased in IL-10-/-mice (%PMN:IL-10-/-=52.8±4.1,control=39.9±1.9*,*p