EFFECT OF ANTIINFLAMMATORY DRUGS ON HUMAN NEUTROPHILS IN ENDOTHELIAL CELL DAMAGE INDUCED BY SHIGA TOXIN-1

VERÓNICA I. LANDONI; PAOLA D'ATRI; M. VICTORIA RAMOS; GABRIELA FERNANDEZ; BÁRBARA REARTE; EVANGELINA A LABORDE; MARINA PALERMO; MIRTA SCHATTNER; MARTÍN A. ISTURIZ

Ginebra

Congreso; XXIst Congress of International Society on Thrombosis and Haemostasis; 2007

The International Society on Thrombosis and Haemostasis

Introduction: Hemolytic Uremic Syndrome (HUS), characterized by hemolytic anemia, thrombocytopenia and acute renal failure, results in renal endothelial damage and is generally caused by E. coli producing Shiga toxins. We had previously demonstrated that the maximal activity of shiga toxin-1 (Stx1) is observed in the presence of inflammatory agents and polymorphonuclear leukocytes (PMN). This may explains the correlation between poor prognosis in HUS and neutrophilia. In this work, we studied the effect of antiinflammatory substances on endothelial cells damage induced by Stx1, inflammatory agents such as lypolysaccharide (LPS) and PMN. Methods: Human umbilical vein endothelial cells (HUVEC) were preincubated with different anti-inflammatory substances and then LPS and/or Stx1 was added. Then, PMN treated with the same antiinflammatory drug were coincubated with the endothelial monolayer. The cytotoxic effect was evaluated by optical microscopy alter crystal violet staining. Results are expressed as percentage of damage ± SD and p