MYELOID IMMATURE CELLS (GR-1+CD11B+) ACQUIRE THEIR INHIBITORY PROPERTIES IN THE BONE MARROW, AND MIGRATE TO LYMPH NODES TO EXERT THEIR FUNCTION

LANDONI, VERÓNICA INÉS; MARTIRE-GRECO, DAIANA; RODRÍGUEZ- RODRÍGUES, NAHUEL; CHIARELLA, PAULA; SCHIERLOH, PABLO; REARTE, BÁRBARA; ISTURIZ, MARTÍN ; FERNÁNDEZ, GABRIELA CRISTINA

Mar del Plata, Provincia de Buenos Aires, Argentina

Congreso; LIX REUNIÓN CIENTÍFICA ANUAL Sociedad Argentina de Investigación Clínica LXII REUNIÓN ANUAL Sociedad Argentina de Inmunología; 2014

SAIC/SAI

Lymph nodes (LN) from LPS-immunosuppressed (IS) micehave a decreased T cell (Ly) proliferation, associated to an incrementof myeloid derived suppressor cells (MDSC, Gr-1+CD11b+).Moreover, MDSC from bone marrow (BM) of IS mice also exhibitedan inhibitory activity towards Ly proliferation. Our aim wasto evaluate whether MDSC acquire their immunosuppressiveproperties in BM and then migrate to LN to exert their function,and evaluate their inhibitory mechanisms. We found that theinhibition of Ly proliferation by BM-IS was accompanied with anincrease in cells expressing inducible nitric oxide (NO) synthase(Gr-1+CD11b+iNOS+) and reactive oxygen species (ROS) (Gr-1+CD11b+DHR+) determined by FACS (p