Influence of Epichloë coenophiala, a seed-borne endophyte, on the micropropagation of tall fescue

REGALADO, JOSE JAVIER; BERDION, VICTORIA; TOSSI, VANESA ELEONORA; IANNONE LEOPOLDO ; PITTA ÁLVAREZ, S.I.; NOVAS, MARÍA VICTORIA

MYCOLOGICAL PROGRESS

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2020

1617-416X

CABE ACLARAR que la fecha que puse es estimativa, tenemos aceptado el paper pero no nos han dicho una fecha de publicacion. La misma es ESTIMATIVA. Copio a continuacion la ACEPTACIÓN DEL EDITOR. Ref.: Ms. No. MYPR-D-20-00124R1Influence of Epichloë coenophiala, a seed-borne endophyte, on the micropropagation of tall fescueMycological ProgressDear Dr. Novas,I am pleased to tell you that your work has now been accepted for publication in Mycological Progress. Thank you for submitting your work to this journal.With kind regardsMarc Stadler, Ph. D. (Dr. rer. nat.)Editor-in-ChiefMycological ProgressABSTRACTAbstractGrasses of the subfamily Pooideae establish mutualistic associations with grass endophyte fungi of the genus Epichloë. These associations are specific, as each host species is associated with one or few Epichloë species. Festuca arundinaceaSchreb. is a perennial C3 species, native to Europe, which has become an important, well-adapted cool season forage crop worldwide. This grass usually establishes symbioses with E. coenophiala, which often benefits the host, but these benefits depend on both plant and fungal genetics and environmental conditions. Micropropagation, allows the development of other biotechnological tools such as genetic transformation, in vitro polyploidization or in vitro endophyte inoculation, therefore, is of interest to improve micropropagation success rate. This is of particular relevance for grasses as they traditionally have been considered as recalcitrant to plant tissue culture. Micropropagation protocols have been published for F. arundinacea, but it was never considered the effect of Epichloë endophytes on the protocol efficiency. The aim of this manuscript was to study the effect of E. coenophiala on the different micropropagation stages of F.arundinacea and on the growth of the regenerated plantlets. The presence ofE. coenophiala(E+) in seeds ofF. arundinacea increased callus induction, enhanced their proliferation and increased shoot regeneration. However, the biomass of plantlets regenerated from E+ seeds was lower than from non-associated seeds, both in vitro and ex vitro, and their in vitro multiplication rate, also decreased. Thus, the presence of E. coenophiala in the seeds did not represent an improvement in the micropropagation of tall fescue.