CONICET | Buscador de Institutos y Recursos Humanos

In vitro studies offer the insights for the understanding of the mechanisms at the tissue?implant interface thatwill provide an effective functioning in vivo. The good biocompatibility of zirconium makes a good candidatefor biomedical applications and the attractive in vivo performance is mainly due to the presence of a protectiveoxide layer. The aim of this study is to evaluate by in vitro and in vivo approach, the influence of surface modificationachieved by anodisation at 30 and 60 V on zirconium implants on the first steps of the osseointegrationprocess. In this study cell attachment, proliferation and morphology of mouse myoblast C2C12-GFP and inmouse osteoprogenitor MC3T3-E1 cells was evaluated. Also, together with the immune system response, osteoclastdifferentiation and morphology with RAW 264.7 murine cell line were analysed. It was found thatanodisation treatment at 60 V enhanced cell spreading and the osteoblastic and osteoclastic cells morphology,showing a strong dependence on the surface characteristics. In vivo tests were performed in a rat femurosteotomy model. Dynamical and static histological and histomorphometric analyses were developed 15 and30 days after surgery. Newly formed bone around Zr60V implants showed a continuous newly compact andhomogeneous bone just 15 after surgery, as judged by the enhanced thickness and mineralization rate. Theresults indicate that anodising treatment at 60 V could be an effective improvement in the osseointegration ofzirconium by stimulating adhesion, proliferation, morphology, newbone thickness and bone mineral apposition,making zirconium an emerging candidate material for biomedical applications.

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