Effect of immunobiotic Lactobacillus rhamnosus nasal treatment on the recovery of T lymphopoiesis in malnourished mice

HERRERA MATÍAS; SALVA SUSANA; BARBIERI NATALIA; AGÜERO GRACIELA; ALVAREZ SUSANA

Buenos Aires

Congreso; LVIII Reunión Anual de la Sociedad Argentina de Inmunología-XIII Jornada Científica del Grupo Rioplatense de Citometría de Flujo-3º Jornadas Argentinas de Inmunodeficiencias Primarias (SAP); 2010

Sociedad Argentina de Inmunología

Thymus produces T cells throughout life but has no self-renewing ability and requires replenishment and recruitment of progenitors derived from the bone marrow (BM). The thymus is a target in malnutrition, leading to the impaired immune response. In this work we evaluated the effect of the nasal administration of Lactobacillus rhamnosus CRL1505 (Lr05) on recovery of T lymphopoiesis in malnourished mice. Weaned mice were malnourished after consuming protein-free diet for 21d. Malnourished mice were repleted during 7d with balanced conventional diet (BD group) or BD with nasal administration of Lr05 (108 cells/d/mouse) on d 6 and 7 (BD+Lr05 group). Well-nourished (WC) and malnourished mice (MC) were used as control. And the end of each treatment we determined body weight, and we studied in BM and thymus the total cells and T cells (CD3, CD4, CD8). Malnutrition induced a significant decrease in total cells and lymphocytes (FSC vs SSC) number in both the BM and thymus (p