INVESTIGADORES
SALVA Maria Susana
artículos
Título:
Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in porcine intestinal epithelial and antigen presenting cells
Autor/es:
VILLENA, JULIO; ERIKO CHIBA; MARIA GUADALUPE VIZOSO-PINTO; YOHSUKE TOMOSADA; TAKUYA TAKAHASHI; TAKAMASA ISHIZUKA; HISASHI ASO; SALVA, SUSANA; ALVAREZ, SUSANA; KITAZAWA, HARUKI
Revista:
BMC MICROBIOLOGY
Editorial:
BIOMED CENTRAL LTD
Referencias:
Lugar: Londres; Año: 2014 vol. 14 p. 126 - 140
ISSN:
1471-2180
Resumen:
Background: Previous findings suggested that Lactobacillus rhamnosus CRL1505 is able to increase resistance of children
to intestinal viral infections. However, the intestinal cells, cytokines and
receptors involved in the immunoregulatory effect of this probiotic strain have
not been fully characterized.
Results: We aimed to gain insight into the mechanisms involved in
the immunomodulatory effect of the CRL1505 strain and therefore evaluated in
vitro the crosstalk between L. rhamnosus
CRL1505, porcine intestinal epithelial cells (IECs) and antigen presenting
cells (APCs) from swine Peyer?s patches in order to deepen our knowledge about
the mechanisms, through which this strain may help preventing viral diarrhoea
episodes. L. rhamnosus CRL1505 was able
to induce IFN?α and ?β in IECs and improve the production of type I IFNs in
response to poly(I:C) challenge independently of Toll-like receptor (TLR)-2 or
TLR9 signalling. In addition, the CRL1505 strain induced mRNA expression of
IL-6 and TNF-α via TLR2 in IECs. Furthermore, the strain significantly
increased surface molecules expression and cytokine production in intestinal
APCs. The improved Th1 response induced by L. rhamnosus CRL1505 was triggered
by TLR2 signalling and included augmented expression of MHC-II and
co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs.
IL-10 was also significantly up-regulated by CRL1505 in APCs.
Conclusions: It was recently reviewed the emergence of TLR agonists
as new ways to transform antiviral treatments by introducing panviral
therapeutics with less adverse effects than IFN therapies. The use of L. rhamnosus CRL1505 as modulator of
innate immunity and inductor of antiviral type I IFNs, IFN-γ, and regulatory
IL-10 clearly offers the potential to overcome this challenge.