FISH-MAPPING OF THE 5S RDNA LOCUS IN CHILI PEPPERS (CAPSICUM-SOLANACEAE)

AGUILERA P.M.; DEBAT, H. J.; SCALDAFERRO, M. A.; MARTÍ, D. A.; GRABIELE, M.

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2016 vol. 88 p. 117 - 125

0001-3765

We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position was found. 5S FISH signals of nearly similar size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid to the cytomolecular characterization of pepper germplasm to evaluate variability and will be instrumental to integrate physical, genetic and genomic maps already generated in the genus.