Modulation of cell matrix adhesion plasticity by bradykinin (BK): a quantitative morphometric study in rat renal papilla collecting duct cells

MÁRQUEZ M. GABRIELA; FERNANDEZ TOME M DEL CARMEN; NORMA STERIN-SPEZIALE

Rosario

Congreso; XLII Reunión de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular (SAIB); 2006

Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular (SAIB)

Focal adhesions (FA) are structures of cell attachment to extracelular matrix. We previously found that BK induces a restructuration of FA by mobilizating FA protein vinculin (V), but not talin (T), by a mechanism that involves phosphatidylinositol 4,5 biphosphate hydrolysis by PLC. Now, we performed a detailed quantitative morphometric analysis of FA, which include size, axial ratio (AR) and average length (AL). After 1?, 5? or 10? (minutes) BK incubation, cultured cells were immunostained and analyzed by confocal microscopy. BK induced a decrease in the number of V-stained FA per cell (Control vs 1?,5?,10?: 72.014.4 vs 25.29.8, 29.49.1, 30.65.1; p