Characterization of an Alkaline/Neutral Invertase in development and abiotic stresses

BATTAGLIA M; LECHNER L; PÉRZ-GARCÍA M; MARTIN M; MARTÍNEZ NOËL G; SALERNO G

Congreso; IPMB2015; 2015

In plants, sucrose (Suc) and its hydrolysis products [glucose (Glc) and fructose (Fru)] have a primary role in carbon metabolism but also as signaling transduction molecules. Invertases (Inv) catalyzed the irreversible hydrolysis to hexosas and are classified by their in vitro optimum pH in acid and alkaline/neutral invertases (A/N-Inv). Recent findings showed the presence A/N-Inv not only in the cytosol but also in organelles, such as chloroplasts and mitochondria. In Arabidopsis, mitochondrial A/N-Inv gene family was represented by three members, named as invA, invC and invH. Interestingly, analysis of knockout mutants in A/N InvA and A/N InvC genes demonstrated a new role for these proteins, showing an impaired growth phenotype with alterations in development and abiotic stress responses. Here, we present the phenotypic analysis of an Ath mutant in A/N-InvH gene growing in normal and under abiotic stress conditions (low temperature, osmotic stress and salinity). Relative expression of plastidic A/N-Inv genes in A/N-InvH mutant background was analyzed as well as a comparison between the other mitochondrial A/N-InvC and A/N-InvA. Our results support the important role that mitochondrial A/N-Inv family members play in plant development and under stress conditions.Supported by CONICET, UNMdP (EXA645), PICT 1288, and FIBA.