INVESTIGADORES
MARTINEZ NOËL Giselle Maria Astrid
congresos y reuniones científicas
Título:
TOR signaling in Arabidopsis thaliana
Autor/es:
SALERNO G; MARTÍNEZ NOËL G
Reunión:
Congreso; SAIB; 2013
Resumen:
Target
of rapamycin (TOR) kinase is an evolutionarily conserved master regulator that
integrates energy, nutrients, growth factors and external signals to promote
survival and growth in eukaryotes. The knowledge of TOR signaling in plants has
been hampered because of the embryo lethality of the Arabidopsis thaliana tor
mutants. The controversial
resistance to rapamycin, a TOR inhibitor in other organisms, limits the
molecular dissection of the TOR pathway in plants. Thus, we analyzed the
effect of second generation inhibitors, such as PP242, in
Arabidopsis plants. Unlike
rapamycin, PP242 is a selective inhibitor that targets ATP domain of TOR. We grew plants in media
with different carbon/nitrogen balance and PP242 concentrations.
The inhibitor was able to delay germination and affected growth plants.
Particularly, root size was severely reduced. Moreover,
we show that the synthesis of anthocyanins and the expression of key genes of
TOR, carbon and nitrogen signaling were modified by the presence of the
inhibitor. Thus, the PP242 inhibitor could be a useful tool to investigate TOR
signaling. This work was supported by CONICET,
ANPCyT, UNMdP and FIBA.