Effect of alendronate on cellular processes involved in atherosclerotic calcifications

CUTINI P.; RAUSCHEMBERGER M.B.; CAMPELO A.; SANDOVAL M.; MASSHEIMER V.

Congreso; XXIX Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral (AAOMM); 2012

Bisphosphonates are effective treatments for osteoporosis and are supposed to inhibit vascular calcifications. Nitric oxide (NO) synthesis, smooth muscle cells (VSMC) migration and monocytes adhesion to endothelial cells (EC) are events involved in vascular calcifications. In this study we investigated whether alendronate (ALN) regulates these cellular through its direct action on vascular cells. To that end, we employed cultures of murine EC and VSMC treated with ALN. The effect of ALN was studied under basal or inflammation conditions induced by the presence of lipopolysacharide (LPS). EC treatment with ALN 5μM (15 min) significantly increased NO production (28.2±2.9 vs 46.3±6.0 nmol NO/mg protein, C vs ALN, p