Effect of estrogen estrone on uterine and adipose tissue under obesity

VALLE M.; CEPEDA S.; CUTINI P.; SANDOVAL M.; MASSHEIMER V.

Congreso; Reunión Anual de Sociedades de Biociencias 2023; 2023

Previously we reported that, estrone (E1) counteracts uterine oxidative stress induced by an inflammatory environment. In this work, using an experimental model of obesity, the direct action of E1 on uterus (Ut) and adipose tissue (AT) was evaluated. In order to rule out the influence of the hormonal impact during estrous cycle, animals were bilaterally ovariectomized (OVX). Rats were fed with high-fat diet (OVX-Ob) for 10 weeks. After isolation, Ut and retroperitoneal AT explants were in vitro expose to 10 nM E1. Histological analysis of Ut explants (H&E staining) showed that in non-OVX rats exhibited a thicker endometrial layer with great amount of uterine glands compared to uterus sections from OVX rats, verifying the absence of estrous cycles in OVX group. The serum biochemical evaluation showed that, Ob rats exhibited higher levels of serum H2O2; TBARS, and leptin than non-obese (8; 65; 122% above non-Ob, respectively, p< 0.05), profile compatible with obesity. After E1 treatment (4h), Ut slices exhibited an enhancement in nitric oxide synthesis (33% above control P