BIOCHEMICAL ACTION OF MEDROXYPROGESTERONE ACETATE ON OVARIECTOMIZED OBESE RATS

CUTINI P.; CEPEDA S.; SANDOVAL M.; MASSHEIMER V.

Congreso; Reunión Anual de Sociedades de Biociencias 2023; 2023

Obesity, cardiovascular and bone diseases are multifactorial clinical entities that often coexist in postmenopausal women. Medroxyprogesterone acetate (MPA) is usually employed in hormone replacement therapy. Previous studies carried out in our laboratory at vascular level, demonstrated a divergent action between native progesterone (Pg) and its synthetic analogue MPA, evidenced by an oxidative, proatherogenic and prothrombotic effect, and an impairment in aortic recovery elicited by MPA in contrast to Pg action. VEGF is a key mediator in vascular and bone repair. The aim of this work was to evaluate the effect of MPA on VEGF production on vascular and bone tissue, as wells as the impact on adiposity oxidative stress in a model of obesity and hypoestrogenism. To that end, aortic endothelial cells (EC), retroperitoneal adipose tissue (AT) and femoral diaphysis (FD) were isolated from ovariectomized Wistar rats fed with a high-fat diet (27%) for 10 weeks. For in vitro treatments, AT explants, FD and EC cultures were incubated with 10 nM MPA for 20 h. Control group received vehicle only (isopropanol). Treatment of EC culture with MPA induced a significant reduction in VEGF synthesis (34% vs control, P