THE BISPHOSPHONATE ALENDRONATE EXERTS A DIRECT ACTION ON ADIPOSE TISSUE IN A MODEL OF HYPOESTROGENISM AND OBESITY

CUTINI P.; CEPEDA S.; SANDOVAL M.; MASSHEIMER V.

Congreso; Reunión Anual de Sociedades de Biociencias 2023; 2023

The expansion of adipose tissue plays a crucial role in the progression of obesity, a chronic disease that generates oxidative stress and an imbalance in the production of proinflammatory adipokines promoting a deleterious impact in many tissues. Bisphosphonates are drugs employed as first line therapy for bone-related diseases, such as postmenopausal osteoporosis. However, several extraosseous effects have been demonstrated in the last decades. Postmenopausal women exhibit a higher prevalence of obesity and overweight, so that, in this work we investigated the impact of the bisphosphonate alendronate (ALN) on adipose tissue in a model of hypoestrogenism and obesity. For this purpose, retroperitoneal adipose tissue was isolated from bilaterally ovariectomized Wistar rats fed with a high-fat diet (27%). For in vitro treatments, slices of adipose tissue (40 mg) were incubated with 5 μM ALN for 18 h. Control group received vehicle only (phosphate buffered saline). Hydrogen peroxide (H2O2), TBARS and leptin levels were measured. Firstly, we detected that serum H2O2, TBARS and leptin levels in obese rats were higher than those detected in normal weight rats (6, 65 and 122% above control, respectively, P