Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling

CUTINI P.; RAUSCHEMBERGER M.B.; MASSHEIMER V.

Congreso; 1º Congreso Argentino de Osteología - XXXIII Reunión Anual de la AAOMM - XII Congreso Argentino de Osteoporosis (CAO); 2016

The genesis of the atheromatous lesion involves the interaction of monocytes and platelets with the endothelium. Finally, vascular calcification takes place through vascular smooth muscle cells (VSMC) transdifferentiation to bone lineage. The impact of bisphosphonates (BP) on cardiovascular diseases remains unknown. The aim of this study was to investigate the role of the BP alendronate (ALN) in cellular and molecular processes involved in vascular disease. Murine cultures cells were used: a) endothelial cells (EC); b) VSMC; c) VSMC induced to osteoblastic transdifferentiation (VSMC-OB) in osteogenic medium (β-glicerolfostato 5 mM; CaCl2 and 4 mM). The effect of ALN on platelet adhesion (PAd) and aggregation (PAg) was investigated. Under basal conditions ALN partially reduced PAd (20–35%; ALN 1–10 μM; p