Vascular response to stress: protective action of the bisphosphonate alendronate

CUTINI P.; CAMPELO A.; MASSHEIMER V.

Congreso; 4º Congreso Argentino de Osteología (XXXVIII Reunión Anual de la AAOMM ? XV Congreso Argentino de Osteoporosis); 2021

Bisphosphonates are first-line drugs in the treatment of postmenopausal osteoporosis, a period in which the prevalence of cardiovascular diseases in women increases significantly. The aim was to study the effect of alendronate (ALN) on the vascular response to extracellular environmental stress. We focused on alterations in cell growth and migration patterns and the capacity for vascular repair by angiogenesis. Primary cultures of endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from murine aorta were used. In order to induce stress conditions, cells were exposed to the pro-inflammatory agent LPS or incubated in a pro-osteogenic medium (OM). Using the MTT assay, we observed that 5 μM ALN totally or partially prevented VSMC proliferation induced by LPS (31% vs control, p