INVESTIGADORES
ESPECHE TURBAY Maria Beatriz
artículos
Título:
beta-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp lactis CRL 581 protects against trinitrobenzene sulfonic acid induced colitis in mice
Autor/es:
ESPECHE TURBAY, B; A. DE MORENO DE LEBLANC; G. PERDIGÓN; G. SAVOY DE GIORI; E. M. HEBERT
Revista:
JOURNAL OF DAIRY SCIENCE
Editorial:
AMER DAIRY SCIENCE ASSOC-ADSA
Referencias:
Lugar: Champaign, Illinois; Año: 2012 vol. 95 p. 1108 - 1118
ISSN:
0022-0302
Resumen:
Lactobacillus delbrueckii ssp. lactis CRL 581, a thermophilic
lactic acid bacterium used as a starter culture
for the manufacture of several fermented dairy products,
possesses an efficient proteolytic system that is able to
release a series of potentially bioactive peptides (i.e.,
antihypertensive and phosphopeptides) from á- and
â-caseins. Considering the potential beneficial health effects
of the peptides released by L. delbrueckii ssp. lactis
lactic acid bacterium used as a starter culture
for the manufacture of several fermented dairy products,
possesses an efficient proteolytic system that is able to
release a series of potentially bioactive peptides (i.e.,
antihypertensive and phosphopeptides) from á- and
â-caseins. Considering the potential beneficial health effects
of the peptides released by L. delbrueckii ssp. lactis
lactic acid bacterium used as a starter culture
for the manufacture of several fermented dairy products,
possesses an efficient proteolytic system that is able to
release a series of potentially bioactive peptides (i.e.,
antihypertensive and phosphopeptides) from á- and
â-caseins. Considering the potential beneficial health effects
of the peptides released by L. delbrueckii ssp. lactis
ssp. lactis CRL 581, a thermophilic
lactic acid bacterium used as a starter culture
for the manufacture of several fermented dairy products,
possesses an efficient proteolytic system that is able to
release a series of potentially bioactive peptides (i.e.,
antihypertensive and phosphopeptides) from á- and
â-caseins. Considering the potential beneficial health effects
of the peptides released by L. delbrueckii ssp. lactisL. delbrueckii ssp. lactis
CRL 581 from milk proteins, the aim of this work was
to analyze the anti-mutagenic and anti-inflammatory
properties of the casein hydrolysates generated by the
cell envelope-associated proteinase of this bacterium.
The ability of á- and â-casein hydrolysates to suppress
the mutagenesis of a direct-acting mutagen 4-nitroquinoline-
N-oxide on Salmonella typhimurium TA 98 and
TA 100 increased concomitantly with the time of casein
hydrolysis. The anti-inflammatory effect of the â-casein
hydrolysate was evaluated using a trinitrobenzene
sulfonic acid (TNBS)-induced Crohn?s disease murine
model. The hydrolysate was administered to mice 10 d
before the intrarectal inoculation of TNBS. The mice
that received â-casein hydrolysate previously to TNBS
showed decreased mortality rates, faster recovery of initial
body weight loss, less microbial translocation to the
liver, decreased â-glucuronidase and myeloperoxidase
activities in the gut, and decreased colonic macroscopic
and microscopic damage compared with the animals
that did not receive this hydrolysate. In addition,
â-casein hydrolysate exerted a beneficial effect on acute
intestinal inflammation by increased interleukin 10 and
decreased IFN-ã production in the gut. Our findings
are consistent with the health-promoting attributes of
the milk products fermented by L. delbrueckii ssp. lactis
TA 100 increased concomitantly with the time of casein
hydrolysis. The anti-inflammatory effect of the â-casein
hydrolysate was evaluated using a trinitrobenzene
sulfonic acid (TNBS)-induced Crohn?s disease murine
model. The hydrolysate was administered to mice 10 d
before the intrarectal inoculation of TNBS. The mice
that received â-casein hydrolysate previously to TNBS
showed decreased mortality rates, faster recovery of initial
body weight loss, less microbial translocation to the
liver, decreased â-glucuronidase and myeloperoxidase
activities in the gut, and decreased colonic macroscopic
and microscopic damage compared with the animals
that did not receive this hydrolysate. In addition,
â-casein hydrolysate exerted a beneficial effect on acute
intestinal inflammation by increased interleukin 10 and
decreased IFN-ã production in the gut. Our findings
are consistent with the health-promoting attributes of
the milk products fermented by L. delbrueckii ssp. lactis
TA 100 increased concomitantly with the time of casein
hydrolysis. The anti-inflammatory effect of the â-casein
hydrolysate was evaluated using a trinitrobenzene
sulfonic acid (TNBS)-induced Crohn?s disease murine
model. The hydrolysate was administered to mice 10 d
before the intrarectal inoculation of TNBS. The mice
that received â-casein hydrolysate previously to TNBS
showed decreased mortality rates, faster recovery of initial
body weight loss, less microbial translocation to the
liver, decreased â-glucuronidase and myeloperoxidase
activities in the gut, and decreased colonic macroscopic
and microscopic damage compared with the animals
that did not receive this hydrolysate. In addition,
â-casein hydrolysate exerted a beneficial effect on acute
intestinal inflammation by increased interleukin 10 and
decreased IFN-ã production in the gut. Our findings
are consistent with the health-promoting attributes of
the milk products fermented by L. delbrueckii ssp. lactis
-oxide on Salmonella typhimurium TA 98 and
TA 100 increased concomitantly with the time of casein
hydrolysis. The anti-inflammatory effect of the â-casein
hydrolysate was evaluated using a trinitrobenzene
sulfonic acid (TNBS)-induced Crohn?s disease murine
model. The hydrolysate was administered to mice 10 d
before the intrarectal inoculation of TNBS. The mice
that received â-casein hydrolysate previously to TNBS
showed decreased mortality rates, faster recovery of initial
body weight loss, less microbial translocation to the
liver, decreased â-glucuronidase and myeloperoxidase
activities in the gut, and decreased colonic macroscopic
and microscopic damage compared with the animals
that did not receive this hydrolysate. In addition,
â-casein hydrolysate exerted a beneficial effect on acute
intestinal inflammation by increased interleukin 10 and
decreased IFN-ã production in the gut. Our findings
are consistent with the health-promoting attributes of
the milk products fermented by L. delbrueckii ssp. lactisL. delbrueckii ssp. lactis
CRL 581 and open up new opportunities for developing
novel functional foods.