Applied of actinobacteria consortia-based bioremediation to restore co-contaminated systems

COLIN VERÓNICA LETICIA; ANTEZANA PABLO EDMUNDO; BOURGUIGNON NATALIA; BENIMELI CLAUDIA SUSANA; FUENTES MARIA SOLEDAD

RESEARCH IN MICROBIOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2023

0923-2508

Global industrialization an d natural resource s extraction has left cocktail s of environmental pollutants. Thus, this work focuses on developing a defined actinobacteria consortium able to restore systems co -contaminated with pollutants occurring in Argentinian environments. In this context, five actinobacteria were tested in soli d medium to evaluate antagonistic interactions and tolerance against lindane (LIN), Reactive Black B – V (RBV), phenanthrene (Ph) an d Cr(VI). The strain s showed absence of antagonism, and most of them tolerated the presence of individual pollutants and their mixtures, except Micromonospora sp. A10. Thus, a quadruple consortium constitute d by Streptomyces sp. A5, M7, MC1, and Amycolatopsis tucumanensis DS M4525 9T, was tested in liquid systems with individual contaminants. The best microbial growth was observed in the presence of RB V and the lowest on Cr(VI). Removals detected were 83.3%, 65.0 % an d 52.4 % for Ph, RB V an d LIN, respectively, with absence of Cr(VI) dissipation. Consequently, the consortium performance was tested against the organic mixture, and a microbial growth similar to the biotic control an d a LI N removal increase (61.2%) were observed. Moreover, the four actinobacteria of the consortium survived the mixture bioremediation process. These results demonstrate the potential of the defined actinobacteria consortium as a tool to restore environments co-contaminated with organic pollutants.