INVESTIGADORES
RODRIGUEZ Valeria Andrea
congresos y reuniones científicas
Título:
Fructose produces oxidative stress and decreases intestinal calcium absorption
Autor/es:
RODRÍGUEZ V; RIVOIRA M; GUIZZARDI S; TOLOSA DE TALAMONI N
Lugar:
Buenos Aires
Reunión:
Congreso; Meeting 2016- Argentine Association of Osteology; 2016
Institución organizadora:
Asociación Argentina de Osteología y Metabolismo Mineral
Resumen:
Fructose (FRU) has important metabolic effects on the liver and has beenassociated with many of the components of metabolic syndrome (MS). Naringin (NA), a natural glycosylated flavonoid, is an antioxidant and has other biological properties. The aim of this study was to determine the effect of FRU, in absence or presence of NA, on the duodenal redox state and the intestinal Ca2+ absorption. Adult male Wistar rats were used: 1) controls, 2) FRU: treated with 10% FRU in the drinking water for 30 consecutive days, 3) FRU + NA: injected daily with NA (40 mg/kg b.w.) for 30 days. Intestinal Ca2+ absorption and Ca2+ uptake in mature enterocytes were measured.Glutathione (GSH), antioxidant enzymes and alkaline phosphatase (AP) activities and duodenal superoxide anion (O2−) were also determined. The weight gain, body mass index, waist circumference and serum triglycerides were significantly higher in FRU rats compared to those from the control rats. These parameters were normalized with NA treatment. The intestinal Ca2+ absorption as the Ca2+ uptake and AP activity decreased in FRU rats in comparison with those from controls. These effects were avoided with NA. FRU rats decreased the GSH content, whereas FRU + NA rats increased GSH content above the control values. FRU rats showed lower SOD and CAT activities than those from the controls, and NA avoided these effects. The levels of O2− increased in the FRU rats and NA prevented this increase. To conclude, MS produced by supplying FRU inhibits the intestinal Ca2+ absorption due, at least in part, by oxidative stress triggering. Since NA has the ability to stimulate the GSH synthesis and to improve intestinal redox state, it can protect against the inhibitory effect of the cation absorption caused by FRU ingestion.
