Breast adenocarcinoma tumorigenesis promotion by overexpression of FASN and HIF1-α induced bypalmitic acid and fructose

FERRERO V; MAZO TM; BAROTTO N; DON J; GRANTON F; MOREIRA-ESPINOZA MJ; RODRÍGUEZ V; PASQUALINI ME

Córdoba

Jornada; XXIV Jornadas de Investigación Científica de la Fac. de Ciencias Médicas de la UNC; 2023

FCM-UNC

Cancer cells are characterized by high cell proliferation within the participation of FASN (fatty acid synthase), catalyzed enzime of fatty acidssynthesis, mainly palmitic acid (PA) (1,2). The activation ofFASNgene is mediated by alpha subunit hypoxia-inducible factor 1 (HIF1-α),increasing the glycolysis in breast cancer (3). We propose to evaluate whether dietary variation in PA and fructose (Fr) regulates FASN andHIF1-α expression in murine breast cancer development.BALB/c mice were divided into 4 dietary groups (n=20c/u), CONTROL (6%corn oil+30%Fr), PCS (20% palm oil+15%Fr), PBA (20% cornoil+45%Fr) and PCS+PBA (20% palm oil+45%Fr). Animals were inoculated at 90 days with murine breast adenocarcinoma cells (LM3: 1x106cells). At 120 days we evaluated: host biochemical parameters, tumor volume, metastasis and necrosis (H/E), lipid profile (GC), FASN andHIF1-α tumor expression (Western Blot:WB, qPCR, IHQ). In vitro: LM3 cells were treated with PA 40uM and Fr 2.5mM. We analyzed viability,cell proliferation (Resazurin and Hoechst), FASN expression (WB) and lipid profile (GC). At least three replicates were performed perexperiment and analyzed by ANOVA(p