Electrophoretic studies on maize endosperm proteins: modifications to Laemmli´s SDS-Page technique

CORCUERA, VR; BERNATENÉ, EA Y NARANJO, CA.

Maize Genetics Cooperation Newsletter

University of Illinois

Lugar: Illinois; Año: 2000 vol. 74 p. 40 - 41

1090-4573

Laemmli´s polyacrylamide gel electrophoresis is based in conditions that assure protein dissociation into their individual polypeptides, minimizing the risk of their aggregation. Electrophoresis is the run, in a discontinuous buffer system using a running buffer with a different pH than the one used to prepare the stacking and resolving gels. These systems were originally developed by Ornstein (1964) and Davis (1964). In our laboratory, electrophoretic studies on maize endosperm proteins were carried out using a Hoeffer Vertical Slab Unit SE 600 and a power source Hoeffer Mighty Slim SX 250. We suggested 8 modifications to the original Laemmli´s technique. The maize samples used belong to different types of endosperm: flint, opaque-2 and waxy. When SDS-PAGE was run according to the above procedures, 19 bands could be observed.