Humanin increases the resistance of breast cancer cells to chemotherapy

GOTTARDO MARÍA FLORENCIA; MORENO AYALA MARIELA A.; IMSEN MERCEDES; ASAD ANTONELA; PIDRE MATIAS LUIS; ROMANOWSKI VÍCTOR; CANDOLFI MARIANELA; SEILICOVICH ADRIANA

Mar del Plata

Congreso; LXI Reunión anual de la Sociedad Argentina de Investigaciones Clínicas; 2016

Humanin (HN) is a mitochondrial derived peptide with potentcytoprotective action in many cell types. It has been described thatendogenous and exogenous HN can protect normal tissues againsttoxic effects of chemotherapy. However, the role of this peptid e intumor development is not well known. We have previously shownthat HN is expressed in murine mammary carcinoma cells (4T1)and exerts antiapoptotic and mitogenic actions against variousinsults such as serum deprivation or TNF-α. The aim of this studywas to investigate whether HN affects the sensitivity of tumor cellsto chemotherapeutic agents. HN (10 μM) hampered the antiproliferativeeffect of doxorubicin (DOXO, 500 nM) on 4T1 cells in vi tro(by ELISA BrdU; C=0.55±0.04; DOXO=0.34±0.02*, HN=0.52±0.02,DOXO+HN=0.53±0.01, *p