Development of cecal predominant microbiota in broilers during a complete rearing using denaturing gradient gel eletrophoresis

BLAJMAN, JÉSICA; ZBRUN, MARÍA; SIGNORINI, MARCELO; ZIMMERMANN, JORGE ALBERTO; ROSSLER, EUGENIA; BERISVIL, AYELÉN PATRICIA; ROMERO SCHARPEN, ANALÍA; ASTESANA, DIEGO; SOTO, LORENA; FRIZZO, LAUREANO

ANIMAL PRODUCTION SCIENCE

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2017 vol. 57 p. 458 - 465

1836-5787

Understanding of the intestinalmicrobiota is crucial to enhance intestinal health and performance parametersin animals. A more exhaustive research of the intestinal microbiota of broilerscould be of interest to implement appropriate intervention measures. The aim ofthe present study was to investigate the development of the predominant cecalmicrobiota in broilers that were fed a Lactobacillus salivarius DSPV 001Pstrain during a complete rearing using denaturing gradient gel electrophoresis(DGGE). Bacterial DNA from cecal samples of 24 broilers at different ages wereamplified by PCR and analyzed by DGGE. A total of 35 DGGE products were excisedand sequenced. Distinctive differences in bacterial communities were observedin the caecum as broilers age. At early stages, identified bacteria within thecaecum of broilers were predominantly Clostridiumrelated species. Also, somesequences had the closest match to the genus Escherichia/Shigella. Furthermore,the caecum was a reservoir rich in uncultured bacteria. The major differenceobserved in our study was an increase of potentially beneficial Lactobacillus atd 45. These results may be attributed to modulation of the microbiota by theprobiotic supplementation. The obtained data could be relevant for futurestudies related to the influence of the microbiota resulting from probioticsupplementation on the performance and the immunological parameters of broilers.